Basics in Medical Microbiology

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Price: $20.00 Date Published: April 5, 2015
Hours: 5.00 School:
Materials: Teacher: Sunita Seemanapalli
Member Rating: Not yet rated

Course Description

This course entitled "Basics in Medical Microbiology" is divided into 5 chapters. The first chapter begins by describing the History of Microbiology, both in a nutshell, and then at length, starting with the invention of the light microscope. Next, the advent of the Electron microscope brought the attention of scientists to the existence of two basic cell types, the prokaryotes and eukaryotes. Therefore, chapters 2 and 3 describe the characteristics of cells and viruses and Microscopy and Staining, respectively. Chapter 4 is the Classification of Microorganisms, including nomenclature. The last chapter of the course, chapter 5, describes the study the growth and nutrition of microbes.

At the end of this course, you will know how the study of Microbes began, who the founding fathers of Microbiology are, you will be able to tell the main differences between prokaryotic and eukaryotic cells, identify the parts of the light microscope and know how they function, you will know how to do Gram staining, which is usually the first step in the identification of a bacteria and is a valuable diagnostic tool in both clinical and research setting. You will also know how to classify microorganisms, based on the unique characteristics they possess; and know how to do the streak plate method, a method used to obtain a pure bacterial culture.

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Course Materials

An initial brief and later lengthy description of the History of Microbiology, including the early lenses made by lens crafters, the discussion over the theory of spontaneous generation and the experiments by Louis Pasteur that led to the refutation of the same theory.

  Material Name Type Size Length
The History of Microbiology in a nutshell Video 18.37 MB 10.40 minutes
The debate over spontaneous generation Video 24.15 MB 14.87 minutes
Prevention, Transmission and Treatment of diseases Video 20.32 MB 11.73 minutes
Golden Age of Microbiology-Louis Pasteur Video 15.67 MB 9.72 minutes
Golden Age of Microbiology - Robert Koch Video 19.49 MB 11.83 minutes
The Second Golden Age of Microbiology Video 14.94 MB 8.90 minutes
The Third Golden Age of Microbiology Video 15.76 MB 9.44 minutes
Test Name Pass Questions
Test 1 70.00% 9

A description of the two basic cell types, the prokaryotes and the eukaryotes and the details of the cell structure for each cell type. A description of viruses, what they are and what they are not and why they are not considered cells.

  Material Name Type Size Length
Prokaryotes -Characteristics, Taxon and External structure Video 33.86 MB 19.27 minutes
Prokaryotes-cytoplasmic membrane, cytoplasm and internal structures Video 20.42 MB 10.88 minutes
Eukaryotes-Characteristics, Classification, Example and Structure Video 9.39 MB 4.91 minutes
Eukaryotes-part 2 Video 26.80 MB 14.38 minutes
Eukaryotes-part 3 Video 10.62 MB 5.79 minutes
Eukaryotes- part 4 Video 11.15 MB 6.33 minutes
Viruses Video 14.08 MB 8.45 minutes
Characteristics of life Powerpoint 1.87 MB  
Test Name Pass Questions
Test 2 90.00% 5

A detailed description of the bright field, electron transmission and electron scanning microscopes apart from the different fluorescent, darkfield and phase contrast microscopes, including parts and light pathways and electron beam pathways.

  Material Name Type Size Length
Comparative sizes Video 17.22 MB 11.50 minutes
Light microscopy-part 1 Video 13.09 MB 7.62 minutes
Light Microscope-part 2 Video 15.41 MB 9.21 minutes
Scanning electron microscope Video 11.28 MB 5.95 minutes
Transmission Electron Microscope Video 7.13 MB 3.87 minutes
Test Name Pass Questions
Test 3 70.00% 10

A detailed description of the steps involved in simple and negative staining as well as Gram-staining and Acid-fast staining including the reasoning behind the use of the primary and secondary dyes and the organisms detected by the staining techniques.

  Material Name Type Size Length
Simple and Negative staining Video 11.91 MB 7.13 minutes
Gram-staining Video 15.46 MB 9.02 minutes
Acid-fast staining Video 20.65 MB 12.20 minutes
Test Name Pass Questions
Test 4 80.00% 4

Beginning with the classification of organisms in general this chapter includes the taxonomy and nomenclature of microorganisms.

  Material Name Type Size Length
Taxonomy Video 20.68 MB 12.02 minutes
Hierarchical system and Nomenclature Video 13.13 MB 7.84 minutes
Phyla in Domains Bacteria and Archea Video 18.30 MB 10.31 minutes
Test Name Pass Questions
Test 5 80.00% 4

This final chapter describes the logarithmic growth of bacteria, the physical and chemical factors that affect microbial growth; the types of cell culture, the cultural characteristics of colonies, and the streak plate method of isolation.

  Material Name Type Size Length
Microbial growth Video 7.02 MB 4.66 minutes
Physical and Chemical factors that affect growth Video 20.11 MB 11.77 minutes
Culture Media Video 24.24 MB 13.47 minutes
The Streak plate method Video 11.50 MB 8.69 minutes
Test Name Pass Questions
Test 6 90.00% 5

Course Reviews

Sunita Seemanapalli

Dr. Sunita Seemanapalli has over 10 years of experience in biomedical research, particularly in field of Microbiology. She has a Medical Degree from the Federal University of Paraiba, Brazil. In order to better understand the basic mechanism of disease processes, she pursued graduate studies in the US. She earned an M.S. from Southern University, Baton Rouge, LA. Her Master's thesis focused on the levels of p53, a tumor suppressor protein in Human Cytomegalovirus-infected cells. She earned her Ph.D. from Louisiana State University, Baton Rouge, LA. Her Ph.D. dissertation focused on the role of Outer Surface Protein C in the pathogenesis of Borrelia burgdorferi, the agent of Lyme disease. In March 2011, Dr. Seemanapalli completed one year of post-doctoral research at Texas A&M, College Station, TX, where she developed mutant and complementation strains of Borrelia burgdorferi expressing virulent genes.

Dr. Seemanapalli worked at Pennington Biomedical Research Center in the Department of Human Genomics for 5 years. She also worked at the Hansen's Disease Center for about a year in research related to multi-drug resistant Mycobacterium tuberculosis.

In reference to her teaching experience, Dr. Seemanapalli taught General Biology Laboratory courses as a Teaching Assistant at Southern University and as an Adjunct Instructor at the Baton Rouge Community College for one and a half years.In the Fall of 2011, Dr. Seemanapalli taught Microbiology lecture and lab course at Blinn College, a Community College located in Bryan, TX.

Dr. Seemanapalli won several awards for excellent powerpoint presentations at local, regional and national scientific meetings. She has a first author and several co-author papers published in several peer-reviewed journals, including in the Journal of Immunology. Her expertise is in the areas of Microbiology, Molecular Biology and Immunology. She is passionate about teaching Microbiology in an engaging and interest-captivating manner.

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